What does PCR stand for and what is its function?

Study for the SACE Stage 2 Biology Exam. Enhance your understanding with quizzes, interactive flashcards, and detailed explanations. Be fully prepared for your exam success!

The correct term is Polymerase Chain Reaction, which is a widely used technique in molecular biology. Its primary function is to amplify specific segments of DNA, making millions of copies of a particular DNA sequence. This amplification is crucial for various applications, including genetic testing, cloning, forensic analysis, and research in genetics and genomics.

The process involves three main steps: denaturation, annealing, and extension. In denaturation, the double-stranded DNA is heated to separate it into two single strands. Then, during the annealing phase, short primers bind to the specific sequences on the single-stranded DNA. Finally, in the extension stage, a DNA polymerase enzyme synthesizes new DNA strands by adding complementary nucleotides to the bound primers. This cycle is repeated multiple times, leading to exponential amplification of the target DNA sequence.

The other options describe incorrect processes or concepts. Protein synthesis is not the main function of PCR, which specifically focuses on DNA amplification rather than proteins. The term "Polymerase Cellular Reaction" does not relate to a recognized biological technique associated with cell division. Similarly, "Protein Chromosome Reorganization" is not an established method for gene editing in the way that PCR functions in DNA analysis and amplification.

Subscribe

Get the latest from Examzify

You can unsubscribe at any time. Read our privacy policy